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Peptide Blend Dosing Math: How Much of Each Peptide Is in a Draw

Every draw from a blend vial carries all the peptides in it, in the same ratio as the vial. Pick an amount for one peptide and the amounts of the others follow. In a 70 mg GLOW vial mixed with 3 mL of bacteriostatic water, 15 units on a U-100 insulin syringe holds 0.5 mg of BPC-157, 0.5 mg of TB-500, and 2.5 mg of GHK-Cu.

8 min read
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Peptide Blends
·By the Peptide Calculator Suite team·Updated

Why a Blend Vial Changes the Math

A single-peptide vial has one number to work out: how many units hold your dose. A blend vial has two or more peptides dissolved in the same water, so they share one concentration ratio. Whatever you draw, each peptide comes along in proportion to how much of it the vial holds.

So you choose the amount of one peptide per draw and the vial sets the rest. If a vial holds five times as much GHK-Cu as BPC-157, every draw has five times as much GHK-Cu as BPC-157, at any water volume. Changing one amount on its own takes separate vials.

The water volume sets how many units a given amount takes, as it does for a single peptide, but adding more or less water leaves the ratio where it was.

What's in GLOW, KLOW, and BPC-157 + TB-500

GLOW and KLOW are the blends people search for by name, and BPC-157 + TB-500 is a common two-peptide vial. They are usually sold with these amounts, but suppliers differ, so go by the amounts printed on your own vial.

Peptide amounts and share of the total in common blend vials
BlendTotalContents (share of vial)Ratio
BPC-157 + TB-50020 mgBPC-157 10 mg (50%)TB-500 10 mg (50%)1 : 1
GLOW70 mgGHK-Cu 50 mg (71.4%)BPC-157 10 mg (14.3%)TB-500 10 mg (14.3%)5 : 1 : 1
KLOW80 mgGHK-Cu 50 mg (62.5%)BPC-157 10 mg (12.5%)TB-500 10 mg (12.5%)KPV 10 mg (12.5%)5 : 1 : 1 : 1

KLOW is GLOW with 10 mg of KPV added. The GHK-Cu stays at 50 mg, so its share of the vial drops from 71.4% to 62.5%, yet each draw still carries five times as much GHK-Cu as BPC-157.

None of these peptides is in an FDA-approved drug. The FDA has raised safety concerns about BPC-157, TB-500, KPV, and injectable GHK-Cu in compounding, citing limited or no human safety data. This blog covers the arithmetic only and makes no claims about what the blends do.

Two Ways to Use the Calculator

The calculator takes one peptide per vial. You can enter a single peptide from the blend or the blend as a whole, and both give the same number of units.

Method 1: enter one peptide. Use that peptide's mg as the vial size and its amount as the dose. The units are right for the whole liquid, because every peptide sits in the same water. For a GLOW vial mixed with 3 mL, enter a 10 mg vial (the BPC-157), 3 mL of water, and 0.5 mg. The calculator returns 15 units.

Method 2: enter the whole blend. Use the total mg as the vial size and scale the dose up to the whole blend. BPC-157 is 10 of GLOW's 70 mg, so 0.5 mg of BPC-157 comes with 3.5 mg of blend. Enter a 70 mg vial, 3 mL, and 3.5 mg. The calculator again returns 15 units.

Units = Peptide amount (mg) × Water (mL) × 100 ÷ Peptide in vial (mg)

Other peptide in the draw = Your amount × (Other mg ÷ Your peptide's mg)

In the GLOW example, 15 units carries 0.5 mg × (50 ÷ 10) = 2.5 mg of GHK-Cu and 0.5 mg × (10 ÷ 10) = 0.5 mg of TB-500. The reconstitution math blog works through the first formula step by step.

How Much of Each Peptide Is in 10 Units

Ten units on a U-100 syringe is 0.1 mL. Amounts scale with the draw, so 20 units holds twice what 10 units holds and 5 units holds half.

Amount of each peptide in 10 units (0.1 mL) of a blend vial mixed with 2 or 3 mL of water
Vial + waterIn 10 units (0.1 mL)
BPC-157 + TB-500 20 mg + 2 mLBPC-157 500 mcgTB-500 500 mcg
BPC-157 + TB-500 20 mg + 3 mLBPC-157 333 mcgTB-500 333 mcg
GLOW 70 mg + 2 mLGHK-Cu 2.5 mgBPC-157 500 mcgTB-500 500 mcg
GLOW 70 mg + 3 mLGHK-Cu 1.67 mgBPC-157 333 mcgTB-500 333 mcg
KLOW 80 mg + 2 mLGHK-Cu 2.5 mgBPC-157 500 mcgTB-500 500 mcgKPV 500 mcg
KLOW 80 mg + 3 mLGHK-Cu 1.67 mgBPC-157 333 mcgTB-500 333 mcgKPV 333 mcg

Moving from 2 mL to 3 mL makes every amount per 10 units a third smaller and keeps the ratio. In a 20 mg BPC-157 + TB-500 vial with 2 mL, 10 units holds 500 mcg of each. With 3 mL it holds 333 mcg of each, so the same 500 mcg needs 15 units.

For draws under 30 units, a 30 unit syringe reads more finely than a 100 unit one. The syringe units blog compares barrel sizes, and the BAC water volume blog covers how the water volume moves a dose along the scale.

Doses Per Vial

All the peptides in a blend run out on the same draw, so the vial holds the same number of doses whichever peptide you count by. The quickest way to get it is from the liquid:

Draws per vial = Water (mL) × 100 ÷ Units per draw

A GLOW vial mixed with 3 mL holds 300 units of liquid. At 15 units a draw that is 20 draws, and dividing any peptide's vial amount by its amount per draw gives the same 20 (10 mg ÷ 0.5 mg of BPC-157, or 50 mg ÷ 2.5 mg of GHK-Cu). A little liquid stays behind in the vial, so the last draw can come up short.

The CDC says to discard an opened multi-dose vial within 28 days unless the manufacturer gives another date. Check whether your draws finish inside that window. Twenty draws taken once a day do; taken twice a week, they would need 10 weeks.

Mistakes From Treating a Blend as One Peptide

The wrong draw usually starts with a blend number in one field and a single-peptide number in the other. With a GLOW vial mixed with 3 mL:

  • A 70 mg vial and a 0.5 mg dose, meant as 0.5 mg of BPC-157, gives 2.1 units. That draw holds 71 mcg of BPC-157, a seventh of the intended amount.
  • A 10 mg vial (the BPC-157) and a 2.5 mg dose, meant as 2.5 mg of GHK-Cu, gives 75 units. That draw holds 12.5 mg of GHK-Cu, five times the intended amount, along with 2.5 mg each of BPC-157 and TB-500.

A vial marked "70 mg" with no breakdown can't be split by peptide at all. Get the per-peptide amounts from the certificate of analysis or the supplier before working out a draw.

Match the Vial Size to the Dose

Use the same peptide for both fields, or the blend total for both. Then write the draw with every peptide in it, for example 15 units = 0.15 mL = 0.5 mg BPC-157 + 0.5 mg TB-500 + 2.5 mg GHK-Cu, and label the mixed vial with its water volume.

Summary

A blend vial fixes the ratio of its peptides, so you can set the amount of one per draw and the rest follow. GLOW is commonly 50 mg GHK-Cu, 10 mg BPC-157, and 10 mg TB-500; KLOW adds 10 mg of KPV. To get the units, enter one peptide's mg and its amount, or the blend total and the matching blend amount, and never mix the two. Every peptide runs out on the same draw, so draws per vial equal the water in mL times 100 divided by the units per draw.

Research & Educational Disclaimer: This blog is published for educational and laboratory reference purposes only. It does not provide medical advice, diagnosis, or treatment instructions. Always verify all calculations independently and consult a qualified healthcare provider.

Sources

  1. Safety Risks Associated with Certain Bulk Drug Substances for Use in Compounding, U.S. Food and Drug Administration. The FDA's safety concerns about BPC-157, TB-500, KPV, and injectable GHK-Cu.
  2. Preventing Unsafe Injection Practices, U.S. Centers for Disease Control and Prevention. The 28-day limit for opened multi-dose vials.